Rapid Communication
Copyright ©2008 The WJG Press and Baishideng.
World J Gastroenterol. Jun 21, 2008; 14(23): 3754-3758
Published online Jun 21, 2008. doi: 10.3748/wjg.14.3754
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Identification of pcDNA3. 1 (+)/FHIT by enzyme-cutting assay. M1: Marker λ/DNA/EcoRI+HindIII; M2: Marker DL2000, lane 1: pcDNA3.1 (+)/FHIT, lane 2: pcDNA3.1 (+)/FHIT post enzyme cutting.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Expression of FHIT mRNA and protein in Hep3B-FHIT, Hep3B-C and Hep3B cells. A: FHIT mRNA; B: FHIT protein. M: 1000 bp marker; Lane 1: Hep3B cells; lane 2: Hep3B-C cells; lane 3: Hep3B-FHIT cells.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Growth curves. A: Growth curves of human hepatocellular carcinoma cells. Luminous absorbance of Hep3B-FHIT, Hep3B-C and Hep3B cells was measured by ELISA (wavelength of 570 nm) after DMSO was added. The measurement was done once a day for 6 d; B: Growth curves of tumors after implantation of Hep3B, Hep3B-C or Hep3B-FHIT cells in nude mice. The mice were injected sc with 1 × 107 (0.15 mL/mouse) Hep3B, Hep3B-C or Hep3B-FHIT cells. After implantation, tumor growth was detected weekly.