Construction and expression of eukaryotic plasmids containing lamivudine-resistant or wild-type strains of Hepatitis B Virus genotype C

Figure 1 A: Electrophoresis of PCR results of HBV genotype C full-length genomes. M: Marker; lanes 1-3: PCR products of YMDD, YIDD and YVDD, respectively; B: Electrophoresis of digestion with EcoRI/HindIII. M: marker; lane 1: pcDNA3.1 (+)/EcoRI; lane 2: pcDNA3.1 (+)/HBV-YMDD/EcoRI/HindIII; lane 3: pcDNA3.1 (+)/HBV-YIDD/EcoRI/HindIII; lane 4: pcDNA3.1(+)/HBV-YVDD/EcoRI/HindIII; C: Electrophoresis of digestion with EcoRI. M: marker; lane 1:pcDNA3.1 (+)/EcoRI; lane 2: pcDNA3.1 (+)/HBV-YMDD/EcoRI; lane 3: pcDNA3.1 (+)/HBV-YIDD/EcoRI; lane 4: pcDNA3.1 (+)/HBV-YVDD/EcoRI.

Figure 2 Sequence analysis of wild-type strain and YMDD mutants (YIDD, YVDD). A: Wild-type strain: 739th base is A; 741th base is G. B: YIDD mutant: 741th base G mutated to T. C: YVDD mutant: 739th base A mutated to G.