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©2008 The WJG Press and Baishideng.
World J Gastroenterol. Apr 28, 2008; 14(16): 2487-2493
Published online Apr 28, 2008. doi: 10.3748/wjg.14.2487
Published online Apr 28, 2008. doi: 10.3748/wjg.14.2487
Figure 1 Western blot analysis of Twist expression in MKN28, pcDNA3 control and TwistS transfectant clones (C1, C2 and C3).
Figure 2 Cell adherence rate, and cell migration and cell invasion ability.
A: Adherence rate in three cell lines; B: Migration ability in three cell lines; C: Invasion ability in three cell lines. aP < 0.05 vs TwistS-MKN28 cells.
Figure 3 Livers from nude mouse models of gastric cancer cell metastasis inoculated with: (A) MKN28; (B) pcDNA3-MKN28; (C) TwistS-MKN28 cells.
Figure 4 Twist promoted Tcf-4 /Lef DNA binding activity with EMSA.
Lane 1: Free probe; Lane 2: Competitor; Lane 3:MKN28 cells; Lane 4:pcDNA3 control cells; Lanes 5-7: Three TwistS clones.
Figure 5 Twist increased expression of cyclin D1 mRNA by RT-PCR.
Lane 1:Marker; Lane 2: MKN28 cells; Lane 3: pcDNA3 control cells; Lanes 4-6: Three TwistS clones.
Figure 6 Western blot analysis in each cell line.
A: Expression of MMP-2 in MKN28 cells, pcDNA3 control cells and TwistAS cells by Western blot analysis; B: Western blot analysis of Twist expression in MKN45, pcDNA3 control and TwistS transfectants clone (C1, C2 and C3).
Figure 7 Down-regulation of Twist-inhibited invasion in MKN45 cells.
Representative photographs of invasion in three cell lines (hematoxylin-eosin staining, × 200 magnification). A: MKN45; B: pcDNA3-MKN45; C: TwistAS-MKN45.
Figure 8 Suppression of Twist-inhibited expression of cyclin D1 mRNA by RT-PCR.
Lane 1: Marker; Lane 2: MKN45 cells; Lane 3: pcDNA3 control cells; Lanes 4-6: Three TwistAS clones.
Figure 9 Comparison of activity of MMP-2 by gelatin zymography assay.
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Citation: Luo GQ, Li JH, Wen JF, Zhou YH, Hu YB, Zhou JH. Effect and mechanism of the
Twist gene on invasion and metastasis of gastric carcinoma cells. World J Gastroenterol 2008; 14(16): 2487-2493 - URL: https://www.wjgnet.com/1007-9327/full/v14/i16/2487.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.2487