Effect of JIANPI HUOXUE decoction on inflammatory cytokine secretion pathway in rat liver with lipopolysaccharide challenge

Figure 1 Effects of JHD on pathological changes and CD68 protein expression and distribution in liver tissue. A: HE stain (× 400); A1: Liver tissue in normal group; A2: Liver tissue in model group; A3: Liver tissue in JHD group; B: CD68 immunohistochemical stain (× 200); B1: Liver tissue in normal group; B2: Liver tissue in model group; B3: Liver tissue in JHD group.

Figure 2 Effect of JHD on serum TNF-α level of rats. A: Normal group; B: Model group; C: JHD group. ^aP < 0.05 vs A; ^cP < 0.05 vs B.

Figure 3 Effect of JHD on protein expression of CD68, p-IκB, TNF-α in liver tissue. A: Normal group; B: Model group; C: JHD group. ^aP < 0.05 vs A; ^bP < 0.01 vs A; ^cP < 0.05 vs B; ^dP < 0.01 vs B.

Figure 4 Effect of JHD on mRNA expression of TNF-α, IL-6, TLR2, TLR4, CD14 in liver tissue. A: Normal group; B: Model group; C: JHD group. ^aP < 0.05 vs A; ^bP < 0.01 vs A; ^cP < 0.05 vs B; ^dP < 0.01 vs B.