Establishment and primary application of a mouse model with hepatitis B virus replication

Figure 1 DNA-dose-dependent HBV replication. Various amount of pHBV4.1 in 1.8 mL saline were injected into BALB/c mice within 5-8 s. Mice were sacrificed on d 4 after injection, and HBV DNA intermediates were detected by Southern blotting.

Figure 2 Time-dependent viral replication after hydrodynamic in vivo transfection with pHBV4. 1. Mice were injected hydrodynamically with 10 μg pHBV4.1 and were sacrificed at different time points after injection. HBV DNA intermediates were detected by Southern blotting.

Figure 3 Detection of HBcAg expression in the liver by immunohistochemical staining (DAB, × 40). Mice were injected hydrodynamically with 10 μg pHBV4.1 and were sacrificed at different time points after injection and HBcAg in mouse liver was detected. A: 1 d after transfection; B: 4 d after transfection; C: 7 d after transfection; D: 10 d after transfection.

Figure 4 Detection of HBsAg expression in liver by immunohistochemical staining (DAB, × 40). Mice were injected hydrodynamically with 10 μg pHBV4.1 and were sacrificed at different time points after injection and HBsAg in mouse liver was detected. A: 1 d after transfection; B: 4 d after transfection; C: 7 d after transfection; D: 10 d after transfection.

Figure 5 Time-dependent HBV antigen expression in serum after hydrodynamic transfection. Mice were injected with 10 μg pHBV4.1. At different time points, HBsAg and HBeAg in the serum were measured by ELISA.

Figure 6 Viral replication in different parts of the mouse liver after hydrodynamic transfection. The results from two mice (A and B) are shown. L: left lobe; R: right lobe; M, middle lobe.

Figure 7 Effect of polyIC on HBV replication in mice. After matched by body weight, age and serum HBeAg, mice were treated with PBS or polyIC. HBV replication levels in the liver of the two groups of mice were compared.