Chromosome 11 aneusomy in esophageal cancers and precancerous lesions- an early event in neoplastic transformation: An interphase fluorescence in situ hybridization study from south India

Figure 1 Representative HE stained sections at × 400 magnification selected for FISH analysis. A: Neoplastic streaks with eosin stained keratin pearls indicating a well-differentiated squamous cell carcinoma; B: Adenocarcinomatous tissue showing columnar epithelial replacement of the normal squamous lining.

Figure 2 Images showing aneusomy with regard to chromosome 11 in esophageal cancer cells. A: Trisomy and tetrasomy chromosome 11 in the same area; B: Pentasomy chromosome 11 with normal disomy.

Figure 3 Axiovision Apotome software generated 3D-image from overlay of 10 consecutive images captured by the Optical sectioning microscopy. A: A portion of the esophageal tissue section from the control showing normal disomy of chromosome 11 (indicated by an arrow); B: Tissues from patients with well-differentiated squamous cell carcinoma (arrow indicating cell with trisomy for chromosome 11).