Anti-tumor activities and apoptosis-regulated mechanisms of bufalin on the orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice

Figure 1 The orthotopic transplantation tumor model of human hepatocellular carcinoma in nude mice on 14 d, tumor size is 0. 5 cm × 0.5 cm.

Figure 2 A: Pathological changes of the orthotopic transplantation tumor cells of NS group in nude mice (× 200); B: Pathological changes of the orthotopic transplantation tumor of BF1 group in nude mice (× 100).

Figure 3 A: Ultrastructural changes of the orthotopic transplantation tumor of NS group in nude mice (scanning electron microscope × 10 000); B: Characteristic apoptosis in the orthotopic transplantation tumor cells of BF1 group in nude mice (scanning electron microscope × 10 000).

Figure 4 Apoptosis in the orthotopic transplantation tumor cells guided by bufalin in nude mice (TUNEL × 100).

Figure 5 A: Nagative expression of bcl-2 in the orthotopic transplantation tumor group of nude mice (× 200); B: Positive expression of bax in the orthotopic transplantation tumor group of nude mice (× 200).

Figure 6 Expression of bax mRNA (A) and bcl-2 mRNA (B) in apoptotic transplanted tumor cells induced by bufalin.