Isolation of Kupffer cells and their suppressive effects on T lymphocyte growth in rat orthotopic liver transplantation

Figure 1 A: Single-layer KCs formed with irregular shape and kidney-like nucleus after 24 h (× 100); B: KCs increased and became prominently larger after 48 h, showing typical macrophage morphologic features with irregular shape, transparent cytoplasm and kidney-like nucleus (× 100).

Figure 2 A: The cells were stained positively for ED2 and the purity was ≥ 90% consistently (× 100); B: The same as Figure 3 (× 400).

Figure 3 Effects of irradiated or non-irradiated KCs on T lymphocyte proliferation. Non-irradiated KCs (W-KC and S-KC) showed significant suppressive effects on T cell proliferation in MLR while irradiated KCs (W-KCi and S-KCi) showed decreased inhibitory effects in MLR.

Figure 4 Suppressive effects of KCs derived from liver allograft on T lymphocyte proliferation. There was a marked decrease of cpm value in MLR when KCs were present in groups D and E, i.e., KCs derived from those two groups had more significantly suppressive effect on T cell proliferation.