Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma

Figure 1 Representative AP-PCR fingerprints of ICC (T) and corresponding normal tissue DNA (N), with primer BC17. Arrowhead indicates the position of altered DNA fragments.

Figure 2 Real-time PCR SYBR Green I fluorescence record versus cycle number of target fragment (518bp) and reference gene (β-globin) in tumor DNA and corresponding normal DNA, in case of DNA amplification (A) and allelic loss (B).

Figure 3 Changes in DNA copy number of ICC (T) and corresponding normal tissue DNA (N) compared with reference gene (β-globin). Case 11 showed no change. Cases 23 and 31 showed loss of DNA copy number, whereas cases 26 and 45 showed DNA amplification at chromosome 17p13.2.

Figure 4 Kaplan-Meier estimated survival rates according to the altered DNA fragment on chromosome 17p13. 2.