A preliminary study of neck-stomach syndrome

Figure 1 Expression of c-Fos in the cervical cord. A: Control group; B: Model group at 3 mo; C: Model group at 5 mo. There were only a few c-Fos expression in the cervical cord of the control group, but an increased c-Fos expression in the model groups at 3 mo and 5 mo after the operation.

Figure 2 Expression of c-Fos in the gastric antrum. A: Control group; B: Model group; C: Enlarged figure of the model group. Neurons were defined as c-Fos-positive when the nucleus was stained with intensity clearly above the faint background stain, whereas neurons were defined as c-Fos-negative when the nucleus was either not stained at all or stained with intensity close to background stain.

Figure 3 Expression of caspase-3 and IL-1β in the cervical cord and stomach. A: An increased immunoreactivity and positive neurons of IL-1β in model group; B: Negative expression of IL-1β in control group; C: An increased expression of caspase-3 in model group; D: Negative immunoreactivity of caspase-3 in control group; E: Positive expression of IL-1β in the stomach of model group; F: Negative expression of IL-1β in the stomach of control group; G: An increased expression of caspase-3 in the stomach of model group; H: Negative immunoreactivity of caspase-3 in the stomach of control group.

Figure 4 Expression of caspase-3 and IL-1β protein detected by Western blot. A: Caspase-3 expression in the cord at 3 mo and 5 mo in different groups; B: IL-1β expression in the cord at 3 mo and 5 mo in different groups; C: Caspase-3 expression in the gastric antrum at 3 mo and 5 mo in different groups; D: IL-1β expression in the gastric antrum at 3 mo and 5 mo in different groups.