Interleukin-1 beta up-regulates tissue inhibitor of matrix metalloproteinase-1 mRNA and phosphorylation of c-jun N-terminal kinase and p38 in hepatic stellate cells

Figure 1 Expression of TIMMP-1 mRNA in HSC detected by RT-PCR. Marker: 200-600 DNA marker; lane 1: control group (without IL-1beta treatment); lane 2: IL-1β group (with IL-1β treatment)

Figure 2 IL-1β activates JNK and p38 in a time-dependent manner in rat HSC. A: Representative Western blot results of JNK and p38; B: Densitometry of Western blot analyzed by Gel-Pro software. n=6. ^bP < 0.01 vs 0 h of JNK, ^aP < 0.05 vs 0 h of p38, ^dP < 0.01 vs 0 h of p38.

Figure 3 Effect of SP600125 and SB203580 on IL-1β-induced expression of TIMMP-1 mRNA in rat HSC. A: Representative photos of different concentrations of SP600125 of RT-PCR; B: Representative photos of different concentrations of SB203580 of RT-PCR. C: TIMMP-1 mRNA expression in A and B. 1: IL-1β + DMSO; 2: IL-1β + SP600125 or SB203580 (10 µmol/L); 3: IL-1β + SP600125 or SB203580 (20 µmol/L); 4: IL-1β + SP600125 or SB203580 (40 µmol/L).n = 6.^aP < 0.05 vs Il-1β+SP600125, ^bP < 0.01 vs IL-1β+SP600125, ^dP < 0.01 vs IL-1β + SB203580.