Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer

Figure 1 A: A representative 2DE gel of a normal tissue. Proteins that become down-regulated in corresponding tumor (Figure 2A) are shown with arrows and capital letters. For a better visualization of spots within the box, silver stained image of another gel is shown; B: Immunodection of actin as an internal control of protein loading, the * represents the location of actin.

Figure 2 A: A representative 2DE gel of tumor tissue. Arrows and numbers indicate up-regulated proteins in comparison with their matched normal tissue (Figure 1A); B: Immunodetection of actin and the * represents the location of actin.

Figure 3 A: Verifying differential expression pattern of ß tropomyosin by RT-PCR in three separate experiments as indicated by numbers (1 to 3), normal versus tumor tissues. The amplification product (431bp) is limited to normal tissue which indicates loss or strong down regulation of this protein as observed by 2DE; B: RT-PCR amplification of ß-actin as an internal control of RT-PCR; C: Electrophoresis of the total RNA from normal and tumor tissues used for cDNA synthesis and RT-PCR. L: DNA marker; N: normal tissue; T: tumor tissue.