Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray

Figure 1 Scatter plots of cDNA microarray analysis. Primary carcinoma cells and lymph node metastatic cells from 58 patients (TNM stage III-IV) with gastric cancer were labeled by Cy-3 and Cy-5 respectively, and hybridized to the cDNA microarray using laser microdissection in combination with microarray.

Figure 2 Immunohistochemical staining of OPCML. A: Normal gastric epithelium showing abundant and strong immunoreactivity; B: Primary gastric cancer demonstrating weak immunoreactivity; C: lymph node metastastasis showing rare immunoreactivity (× 400).

Figure 3 Validation of microarray data by semiquantitative RT-PCR. A: Expression levels of 3 genes (SLC9A1, OPCML and UBAP) was up-regulated in primary carcinoma; B: Expression of 3 genes (COX6C, SERPINB1 and CCL22) was down-regulated in primary carcinoma. C: A sample from the primary carcinoma cells; L: A sample from the metastatic gastric carcinoma cells.

Figure 4 Identification of differentially expressed genes in progrssive gastric cancer by semiquantitative RT-PCR. N: A sample from normal gastric epithelium; C: Signifies a sample from the primary carcinoma cells; and L: A sample from the metastatic carcinoma cells.