Hepatitis B virus genotypes in chronic liver disease patients from New Delhi, India

Figure 1 Restriction Digestion of 485 bp PCR products into two fragments by Nla IV. M: Hae III digested Phi X 174 DNA ladder, Lanes 1- 4: Restriction Digestion of 485 bp into 265 bp and 186 bp fragments showing the presence of Genotype D; lanes 5,6: Restriction Digestion of 485 bp into 265 bp, 220 bp and 186 bp fragments showing the presence of mixed Genotypes A and D; lanes 7: Restriction digestion of 485 bp into 265 bp and 220 bp fragments showing the presence of genotype A.

Figure 2 Agarose gel electrophoresis of HBV genotypes A and D, (Using Mix-A, Mix-B, PCR with type-specific primers based genotyping strategy). PCR Product of 119 base pairs (type D) and 68 base pairs (type A). Lane M- (Phi X 174Hae III) Molecular weight marker, lane 1, 3- Negative Control, lane2-Positive Controls, lanes 4-8: Samples; Lanes 4, 5, 8: Type D; Lanes 6, 7: Type A.