Effects of IκBα and its mutants on NF-κB and p53 signaling pathways

Figure 1 Microscopy of YFP-p65 and p53-DsRed in living cells.

Figure 2 Localization patterns of CFP-IκBα, CFP-IκBαM, CFP-IκBα243N and CFP-IκBα244C in living cells.

Figure 3 Amplification plots of five fold serial dilutions of β-actin cDNA. The fluorescence values versus cycle number are displayed.

Figure 4 Standard curve constructed with the β-actin cDNA standards from 1. 00E + 1 to 3.20E + 3 by plotting the logarithmic concentration of the standard versus the crossing points (cycle number).

Figure 5 Effects of IκBα and its mutants on NF-κB and NF-κB downstream gene TNF-α. Abscissa showed different transfected Cells. Y-coordinate expressed the target/reference ratio of the samples divided by the tar = get/reference ratio of the control. In all experiments β-actin cDNA was reference. (^aP < 0.05 vs control).

Figure 6 Effects of IκBα on over expressed NF-κB. (^aP < 0.05 vs control; ^cP < 0.05 vs YFP-p65/CFP-C1 group).

Figure 7 Effects of IκBα and its mutants on p53 and p53 downstream gene Bax. (^aP < 0.05 vs control).

Figure 8 IκBα244C and p53 synergistically mediates apoptosis. (^aP < 0.05 vs control; ^bP < 0.01 vs p53-DsRed/CFP-IκBα244C group).

Figure 9 IκBα participates in NF-κB and p53 signaling pathways.