Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis

Figure 1 Analysis of RT-PCR amplification of IL-18 in PBMC of CP patients.

Figure 2 IL-18 in cell lysates of PBMC and in pancreatic tissue of CP patients. A: In the pancreatic tissue; B: In cell lysates of PBMC.

Figure 3 In vitro IL-18 secretion from PBMC of CP patients. ^aP < 0.0007 25 μg/L LPS vs unstimulated; ^bP < 0.0007 250 μg/L LPS vs unstimulated; ^cP < 0.0007 2500 μg/L LPS vs unstimulated; ^dP < 0.0001 Ethanol/25 μg/L LPS vs 25 μg/L LPS; ^eP < 0.0001 Ethanol/250 μg/L LPS vs 250 μg/L LPS; ^fP < 0.0001 Ethanol/2500 μg/L LPS vs 2500 μg/L LPS.

Figure 4 IL-18 expression in pancreatic tissue of CP patients. A: Clusters of infiltrating mononuclear cells stained positive; B: Clusters of pancreatic acinar cells also stained positive for the expression of IL-18; C: No positive staining for IL-18 in the negative control (mouse serum with IL-18 antigen-antibody mixture).