Detection of YMDD mutation using mutant-specific primers in chronic hepatitis B patients before and after lamivudine treatment

Figure 1 Sensitivity and specificity of the YMDD mutant detection by mutant-specific PCR. A: Various copies of wild-type sequence, YVDD mutant sequence, and mix of both sequences at the indicated concentrations. The left most lane contains molecular weight marker in base pair unit. The detection limit was 10¹ copies of YVDD mutant in 10⁸ copies of wild-type HBV or 10⁴ copies of YVDD mutant in 10⁹ copies of wild-type HBV; B: Various copies of wild-type sequence, YIDD mutant sequence, and mix of both sequences at the indicated concentrations. The left most lane contains molecular weight marker in base pair unit. The detection limit was 10¹ copies of YIDD mutant in 10⁸ copies of wild-type HBV or 10³ copies of YIDD mutant in 10⁹ copies of wild-type HBV.