Synergistic effect of a novel oxymatrine-baicalin combination against hepatitis B virus replication, α smooth muscle actin expression and type I collagen synthesis in vitro

Figure 1 The inhibition rate of OB on HBsAg and HBeAg secretion in the 2. 2.15 cell culture system. n = 3, mean ± SD. ^aP < 0.05 vs oxymatrine.

Figure 2 The effect of OB on the HBV DNA level in the 2. 2.15 culture system. n = 3, mean ± SD. ^aP < 0.05, ^bP < 0.01 vs control, ^cP < 0.05 vs oxymatrine.

Figure 3 Effect of OB treatment on the α SMA mRNA level in HSC-T6 cells. The α SMA mRNA level was determined by semi quantitative RT-PCR analysis and corrected by β actin mRNA level. n = 3, mean ± SD. ^aP < 0.05, ^bP < 0.01 vs control, ^cP < 0.05 vs oxymatrine.

Figure 4 Effect of OB treatment on the α SMA protein level in HSC-T6 cells. The α SMA protein level was determined by Western blot analysis and corrected by β actin protein level. n = 3, mean ± SD. ^bP < 0.01 vs control, ^aP < 0.05 vs oxymatrine.

Figure 5 Effect of OB treatment on the Collagen I mRNA level in HSC-T6 cells. The collagen mRNA level was determined by semi quantitative RT-PCR analysis and corrected by the β actin mRNA level. mean ± SD. ^bP < 0.01 vs control, ^dP < 0.01 vs oxymatrine.

Figure 6 Effect of OB treatment on the collagen I protein level in HSC-T6 cells. The collagen protein level was determined by Western blot analysis and corrected by the β actin protein level. n = 3, mean ± SD. ^bP < 0.01 vs control, ^dP < 0.01 vs oxymatrine.