Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 21, 2006; 12(31): 4996-5004
Published online Aug 21, 2006. doi: 10.3748/wjg.v12.i31.4996
Figure 1
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Figure 1 Th1/Th2-like pattern of gene expression in the ileal mucosal layer after γ-irradiation in rats. (■): CAPE-untreated, (□): CAPE-treated and CD4+ immunostaining in control (C), 6 h and 7 d (D7) after irradiation (D (x 20) (mean ± SEM, n = 6); aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.001 vs untreated.
Figure 2
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Figure 2 Expression of the chemokine receptors CXCR3/CCR5 and CCR4 post-irradiation in rats. (■): CAPE-untreated; (□): CAPE-treated (mean ± SEM, n = 6); aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs untreated rats.
Figure 3
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Figure 3 Expression of transcription factors of Th1/Th2 polarization after irradiation in rats. (■): CAPE-untreated; (□): CAPE-treated. A: Western blot analysis using T-Bet and GATA-3 polyclonal antibody, GAPDH levels are used as internal standard; B: Real-time RT-PCR analysis (mean ± SEM, n = 6); aP < 0.005, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs untreated rats.
Figure 4
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Figure 4 Expression of the regulator cytokine gene SOCS1 and SOCS3 after irradiation in rats. (■): CAPE-untreated; (□): CAPE-treated (mean ± SEM, n = 6); aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs untreated.
Figure 5
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Figure 5 Irradiation effect on extrinsic apoptotic factors TNF-α, FasL and related-receptors expression post-irradiation in rats. (■): CAPE-untreated; (□): CAPE-treated (mean ± SEM, n = 6); aP < 0.05, bP < 0.01 vs control; cP < 0.05, dP < 0.01 vs untreated rats.
Figure 6
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Figure 6 Irradiation effect on intrinsic apoptotic factor expression modulation and caspase activity post-irradiation in rats. (■): CAPE-untreated (□): CAPE-treated. Apoptotic cells presence was confirmed by the terminal deoxynucleotidyltransferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) staining in ileum. (A): Control; (B): 6 h; (C): 7 d after γ-irradiation. (D): 6 h; (E): 7 d from irradiated CAPE-treated (x 20) (mean ± SEM, n = 6); bP < 0.01 vs control; dP < 0.01 vs untreated.