Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells

Figure 1 After 3 passages, on the 14^th d, the cell showed uniform morphology: fusiform shape or polygon shape, like fibroblast and grew as grass bunch or whirlpool (100 ×).

Figure 2 Growth condition of MSC cultured with cholestatic serum. A: Cultured with cholestatic serum for 7 d, peripheral cell of the clone showed cuboidal morphology, which is characteristic of hepatocytes (100 ×); B: Cultured with cholestatic serum for 35 d, all MSCs showed cuboidal morphology, which is characteristic of hepatocytes (100 ×).

Figure 3 Growth condition of MSC cultured with HGF. A: Cultured with HGF for 7 d, peripheral cell of the clone showed cuboidal morphology, which is characteristic of hepatocytes(100×); B: Cultured with HGF for 35 d, all MSCs showed cuboidal morphology, which is characteristic of hepatocytes (100 ×).

Figure 4 MSC identification. A: Induced by vitamin C, dexamethasone and β sodium glycerophosphate, MSC differentiated into osteoblast (200×); B: Alkaline phosphatase stain showed positive (400 ×); C: Alkaline phosphatase stain negative control (100 ×).

Figure 5 Expression of CK18 and AFP. A: Cultured with cholestatic serum for 14 d, MSC expressed AFP by S-P method (100×); B: Cultured with HGF for 14 d, MSC expressed CK18 by S-P method (100 ×); C: Negative control (100 ×).

Figure 6 Expression of albumin. A: Culture with cholestatic serum for 35 d, expression of albumin was confirmed by in situ hybridization (FITC dyeing ) (400 ×); B: Culture with HGF for 35 d, expression of albumin was confirmed by in situ hybridization (FITC dyeing ) (400 ×).