Formation of microchimerism in rat small bowel transplantation by splenocyte infusion

Figure 1 Heterotopic small bowel transplantation in rats. The graft vasculature was anastomosed. TSB: transplanted small bowel; RSB: recipient’s small bowel.

Figure 2 Agarose gel electrophoresis of PCR pro-ducts. M: 2 Kb DNA ladder; lane 1: PCR amplification bands from normal SD rats; lane 2: PCR amplification bands from allotransplantation group rats; lane 3: PCR amplification bands from allotransplantation + spleno-cyte infusion group rats.

Figure 3 Histopathological findings in different groups after SBT. A: Few histopathological changes in the normal tissue (group 3); B: Intact villi and some infiltrates in allografts obtained from donor splenocyte recipients (group 2); C: Inflammatory infiltrates throughout the intestine and extensive apoptosis (arrows) and ulceration in the transplanted intestine (group 1).