Expression of T-STAR gene is associated with regulation of telomerase activity in human colon cancer cell line HCT-116

Figure 1 A: The quantitation of T-STAR gene cDNA by RT-PCR from mRNA on electrophoresis of agarose gel; B: The relative expression levels of T-STAR mRNA of each group on electrophoresis of agarose gel, ^aP < 0. 01 vs 2. M: Marker; 1: HCT; 2: HCT-pcDNA; 3: HCT-asSTAR; 4: HCT-STAR.

Figure 2 A: Western blot analysis of the T-STAR protein expression level of each group of cells; B: Densitometry analysis of the amount of T-STAR protein expression in each group of cells, ^aP < 0. 01 vs 2. 1: HCT; 2: HCT-pcDNA; 3: HCT-asSTAR; 4: HCT-STAR.

Figure 3 Quantitation analysis of telomerase activity of each HCT cell groups, ^aP < 0. 05 vs 2, ^bP < 0.01 vs 2. 1: HCT; 2: HCT-pcDNA; 3: HCT-asSTAR; 4: HCT-STAR.