Apoptosis of human colon carcinoma HT-29 cells induced by ceramide

Figure 1 Morphology change of apoptosis in HT-29 cells induced by C₂-ceramide at different concentrations for different time under transmission electron microscope. A: 0 μmol/L, 24 h, × 5000; B: 50 μmol/L, 24 h, × 4000; C: 50 μmol/L, 48 h, × 3000.

Figure 2 Morphology change of apoptosis in HT-29 cells induced by C₂-ceramide at different concentrations for different time under fluorescence microscope. A: 0 μmol/L, 24 h, × 100; B: 50 μmol/L, 12 h, × 100; C: 50 μmol/L, 24 h, × 100).

Figure 3 DNA fragmentation induced by C₂-ceramide in HT-29 cells for 12 h (A) and 24 h (B). 1: 50 μmol/L; 2: 25 μmol/L; 3: 12.5 μmol/L; 4: 0 μmol/L.

Figure 4 Expression of apoptosis-related genes in HT-29 cells induced by C₂-ceramide for 24 h (C: 0 μmol/L; L: 12. 5 μmol/L; M: 25 μmol/L; H: 50 μmol/L).