Down-regulation of survivin expression by small interfering RNA induces pancreatic cancer cell apoptosis and enhances its radiosensitivity

Figure 1 Reports of the sequencing of plasmid Pgenesil-sur(+) (A) and plasmid Pgenesil-sur (-) (B).

Figure 2 Expression of EGFP in PC-2 cells 24 h after trancfection of plasmid Pgenesil-sur(+) (A) and plasmid Pgenesil-sur(-) (B) (original magnification×200).

Figure 3 Down-regulation of survivin mRNA expression detected by semi-quantitive RT-PCR. 1: The blank control group; 2: The negative control group; M: Marker I; 3: The positive experiment group.

Figure 4 Down-regulation of survivin expression detected by immunohisto- chemical SP method in the blank control group (A), negative control group (B), and positive experiment group (C) (coloration with DAB, original magnification × 400)

Figure 5 Apoptosis of PC-2 cells detected in 6 groups by flow cytometry (stained with PI)