Wnt/β-catenin signaling pathway is active in pancreatic development of rat embryo

Figure 1 (A-C): Representative RT-PCR results of β-catenin, APC and cyclin D1 genes in embryonic pancreas of E14. 5-E18.5 and adult rat. A: RT-PCR results of β-catenin gene (low) and ββ-actin gene (top, same in other lanes); B: RT-PCR results of APC gene (low); C: RT-PCR results of cyclinD1 gene (low). M: Molecular -weight marker, lane 1-4: amplification result of RT -PCR for β-catenin, APC and cyclin D1 genes from embryonic pancreas of E14.5, 16.5, 18.5 d and adult rat, respectively.

Figure 2 (A-D) Representative immunohistochemical results of β-catenin, APC and cyclin D1 proteins in embryonic pancreas of E14. 5-E18.5 and adult rat. Immunohistochemical staining was performed using an anti-β-catenin APC and cyclin D1antibodies respectively. Slides were detected at ×400 magnification under a light microscope. A: showed staining of β-catenin at cytoplasm (black arrow) and nucleus (red row) in embryonic pancreas of E18.5. B: showed staining of cyclin D1 within cytoplasm (black arrow) and/or nucleus (red row) in embryonic pancreas of E16.5. C: showed staining of APC within cytoplasm (black arrow) in embryonic pancreas of E18.5. D: showed staining of APC within cytoplasm (black arrow) in adult rat pancreas.