A comparison of gene expression in mouse liver and kidney in obstructive cholestasis utilizing high-density oligonucleotide microarray technology

Figure 1 Confirmation of microarray data by Northern analysis of selected genes. Northern blots were performed with aliquots of pooled RNA from livers and kidneys from each 4 pair-fed sham-operated and 4 bile duct ligated mice, 7 d after surgery. Cyp7b1: Cytochrome P450 7b1; Oct1: Organic cation transporter 1; Spmt: Similar to putative integral membrane transport protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Sham: Sham-surgery; BDL: Bile duct ligation.

Figure 2 Indirect immunofluorescence of organic cation transporter 1 in murine liver sections. A: A low magnification view (x 20) shows antibody labeling at the basolateral membranes of hepatocytes of the pericentral zone of the liver lobule in the liver section of a sham-operated mouse, 7 d after surgery; B: In contrast, there is only a weak signal for organic cation transporter 1 after bile duct ligation.