Expression and activity of inducible nitric oxide synthase and endothelial nitric oxide synthase correlate with ethanol-induced liver injury

Figure 1 Representative pathologic changes in 4-wk dextrose group (A), 4-wk ethanol group (B), and 6-wk ethanol group (C). Original magnification, ×200.

Figure 2 Expression of NF-κB p65 in 6-wk dextrose group (A), 6-wk ethanol group (B) and the number of positive cells in high-power fields (C). ^aP < 0.01 vs dextrose group. Original magnification, ×400.

Figure 3 Immunohistochemical detection of iNOS in 6-wk dextrose group (A), 4-wk ethanol group (B), and 6-wk ethanol group (C). Original magnification, ×200.

Figure 4 RT-PCR analysis of mRNAs for iNOS, eNOS and TNF-α in the liver. A: representative bands for iNOS, TNF-α, eNOS and GAPDH transcripts (lane 1: 6-wk dextrose group, lane 2: 4-wk dextrose group, lane 3: 6-wk ethanol group, lane 4: 4-wk ethanol group); B: normalized densitometric ratios of iNOS, TNF-α and eNOS transcripts to GAPDH. ^aP < 0.01 vs dextrose group.