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©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Feb 7, 2005; 11(5): 756-759
Published online Feb 7, 2005. doi: 10.3748/wjg.v11.i5.756
Published online Feb 7, 2005. doi: 10.3748/wjg.v11.i5.756
Figure 1 Degested results of the empty rector pSuNeo and recombinant pSuNeo-SVV.
A: Electrophoretic profile of pSuNeo-SVV digested with restriction enzymes. Lane 1: DNA marker DL-15000, lane 2: pSuNeo, lane 3: pSuNeo/Sal I, lane 4: pSuNeo-SVV, lane 5: pSuNeo-SVV/Sal I, lane 6: pSuNeo-SVV/Sca I. B: Electrophoretic profile survivin gene by RT-PCR. Lane 1: DNA marker DL-2000, lane 2: untransfected survivin gene, lane 3: pSuNeo, lane 4: pSuNeo-SVV, lane 5: empty control, lane 6: β-actin.
Figure 2 Immunohistochemical staining of survivin in SMMC-7721 cells.
A: SMMC-7721 cells, B: SMMC-7721 cells transfected with pSuNeo, C: SMMC-7721 cells transfected with pSuNeo-SVV.
Figure 3 SMMC-7721 cells transfected with pSuNeo-SVV (A) and pSuNeo (B).
Figure 4 Growth curve of SMMC-7721 cells.
- Citation: Cheng SQ, Wang WL, Yan W, Li QL, Wang L, Wang WY. Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721. World J Gastroenterol 2005; 11(5): 756-759
- URL: https://www.wjgnet.com/1007-9327/full/v11/i5/756.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i5.756