Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis

Figure 1 RT-PCR analysis of PMNs PECAM-1. A: Expression of on PMNs PECAM-1 mRNA in peripheral circulation. Lanes 1, 2, 3 and 4: PECAM-1 cDNA after amplification from RNA of PMNs from inferior vena cava at 2, 4, 6 and 8-h time points of AEP respectively; B: Expression of on PMNs PECAM-1 mRNA on in pancreatic microcirculation. Lanes 1, 2, 3 and 4: PECAM-1 cDNA after amplification from RNA of PMNs from splenic vein at 2, 4, 6 and 8-h time points of AEP respectively; C: Values of the density of PECAM-1 RT-PCR product normalized to that of β-actin in the same RNA sample. Symbols are: (ν) peripheral circulation; (ο) pancreatic microcirculation. ^aP<0.05, by one-way ANOVA.

Figure 2 Flow cytometry analysis of PECAM-1 on PMNs. ^aP<0.05 vs peripheral circulation values.