Increased expression of mitogen-activated protein kinase and its upstream regulating signal in human gastric cancer

Figure 1 Assessment of ERK-1, ERK-2, ERK-3, p38 and MEK-1 expression by Western blot. A: Expression of ERK-1 in gastric carcinoma; B: Expression of ERK-2 in gastric carcinoma; C: Expression of ERK-3 in gastric carcinoma; D: Expression of p38 in gastric carcinoma; E: Expression of MEK-1 in gastric carcinoma. Rabbit polyclonal anti-ERK-1, anti-ERK-2, anti-ERK-3, anti-p38 and anti-MEK-1 antibodies were used to recognize the 44 ku, 42 ku, 63 ku, 41 ku, and 41 ku isoforms respectively.

Figure 2 Assessment of MEK-1 expression in human gastric cancer, adjacent normal mucosa, and metastatic lymph nodes by immunohistochemical staining. Magnification, A-C, ×200; D, ×100. A: Expression of MEK-1 in primary sites; B: Expression of MEK-1 in normal mucosa; C, D: Expression of MEK-1 in metastatic lymph nodes.