Cyclosporine A, FK-506, 40-0-[2-hydroxyethyl]rapamycin and mycophenolate mofetil inhibit proliferation of human intrahepatic biliary epithelial cells in vitro

Figure 1 Freshly isolated (A) and proliferating human intrahepatic BECs (B) as well as BECs exposed to immunosuppressive agents (C).

Figure 2 Flow cytometry analysis of CK19 expression. A: negative control. B: with antibody against CK19.

Figure 3 MTT analysis of proliferation of BECs exposed to drugs. A: BECs exposed to the drugs for 24 and 48 h. At 48 h, CsA (500 µg/L), FK-506 (100 µg/L), RAD (0.25 µg/L), and MMF (100 µg/L) inhibited proliferation of BECs (P<0.05). B: BECs exposed to the drugs for 48 h. CsA, FK-506, RAD, and MMF inhibited the proliferation of BECs in a dose-dependent manner.

Figure 4 Western blot analysis of CK19 expression. BECs were treated with 500, 1 000, 2 000 µg/L CSA, and FK-506, respectively for 48 h. CsA and FK-506 did not change the protein expression of CK19 in BECs.