Effect of pseudolaric acid B on gastric cancer cells: Inhibition of proliferation and induction of apoptosis

Figure 1 Chemical structure of pseudolaric acid B (C₂₃H₂₈O₈, MW = 432. 5).

Figure 2 Dose- and time-dependent inhibitory effect of pseudolaric acid B on growth of AGS cells. A: AGS cells were treated with various doses of pseudolaric acid B for 24 h; B: AGS cells were treated with 5 μmol/L pseudolaric acid B for various time periods. Each point indicates mean±SD of three independent experiments.

Figure 3 Time-dependent effect of 5 μmol/L pseudolaric acid B on morphological changes in AGS cells. A: 0; B: 12; C: 24; D: 48 h.

Figure 4 Time-dependent effect of 5 μmol/L pseudolaric acid B on internucleosomal DNA fragmentation in AGS cells. M: Marker; lane 1: 0 h; lane 2: 12 h; lane 3: 24 h; lane 4: 48 h.

Figure 5 cdc2 levels in AGS cells down-regulated by pseudolaric acid B treatment.

Figure 6 Time-dependent down-regulation of Bcl-2, activation of caspase-3, and PARP cleavage in cultured AGS cells induced by pseudolaric acid B.