Effect of oral Lactococcus lactis containing endostatin on 1, 2-dimethylhydrazine-induced colon tumor in rats

Figure 1 Experimental design.

Figure 2 Expression plasmid obtained by restriction enzyme analysis. P: expression plasmid cut by EcoRI and Not I; e: endostatin gene; M: molecular weight marker.

Figure 3 Expression of rat endostatin gene in L lactis. A: Silver-strained SDS-PAGE of expressed endostatin in L lactis; B: Western blot. Lanes 1-6: engineered L lactis 1-6 h after induction; lane 7: L lactis without endostatin gene; lane 8: rat sera from groups after oral recombinant L lactis; lane 9: endostatin protein expression in E coli cells.

Figure 4 Survival rate of rats injected with DMH with or without endostatin and normal saline.