Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jan 28, 2005; 11(4): 545-550
Published online Jan 28, 2005. doi: 10.3748/wjg.v11.i4.545
Figure 1
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Figure 1 Freshly isolated islets of Langerhans from Syrian golden hamsters. The smooth appearance of the islets’ shape, and the variation in size could be observed. Magnification ×120.
Figure 2
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Figure 2 Core cell damage of isolated islets after 2-d culture at 37 °C in 950 mL/L O2/50 mL/L CO2 atmosphere. The core cell damage occurred predominantly in the larger islets. Magnification ×70.
Figure 3
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Figure 3 HE staining of isolated islets after 2-d culture at 37 °C in 950 mL/L O2/50 mL/L CO2 atmosphere (Magnification ×100). The core β-cell damage occurred within these larger islets.
Figure 4
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Figure 4 TUNEL assay of isolated islets after 2-d culture at 37 °C in 950 mL/L O2/50 mL/L CO2 atmosphere (Magnification ×120). TUNEL-positive cells containing damaged DNA developed a dark brown color in the nuclei, indicating cell apoptosis.
Figure 5
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Figure 5 Intact histological structure of isolated islets after a 7-d culture at 26 °C in 950 mL/L O2/50 mL/L CO2 atmosphere (H-E staining). Magnification ×150.
Figure 6
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Figure 6 Core cell damage in re-warmed islets with diameters <100 μm (A), 100-200 μm (B), 200-300 μm (C), and >300 μm (D) after a 2-d culture preconditioning at 26 °C during a 7-d culture at 37 °C. Islets cultured only at 37 °C served as controls. The 2-d culture preconditioning at 26 °C did not prevent core cell damage in islets with diamerer >100 μm under 37 °C culture conditions.
Figure 7
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Figure 7 Core cell damage in rewarmed islets with diameters <100 μm (A), 100-200 μm (B), 200-300 μm (C), and >300 μm (D) after a 4-d culture at 26 °C during a 7-d culture at 37 °C. Islets cultured only at 37 °C served as controls. The 4-d culture preconditioning at 26 °C did not prevent core cell damage of islets with diameter>100 μm under 37 °C culture conditions.
Figure 8
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Figure 8 Re-warmed islets with diameters <100 μm (A), 100-200 μm (B), 200-300 μm (C), and >300 μm (D) after a 7-d culture at 26 °C during an additional 7-d culture at 37 °C. Islets cultured only at 37 °C served as controls. The 7-d culture preconditioning at 26 °C was effective to reduce core cell damage of islets with diameter >100 μm under 37 °C culture conditions. aP<0.05 vs Controls.