Separation of growth-stimulating peptides for Bifidobacterium from soybean conglycinin

Figure 1 Chromatography of soybean conglycinin on sepharose-CL-6B. Bed size,1 cmx100 cm, flow rate 30 mL/h, absorbance at 280 nm.

Figure 2 SDS-PAGE pattern of purified conglycinin. a, a and b indicate subunits of conglycinin. M: standard molecular weight proteins.

Figure 3 Chromatography of soybean conglycinin hydrolysates on sephadex-G15. Bed size, 1.0 cmx100 cm, flow rate 15 mL/h, absorbance at 280 nm.

Figure 4 MALDI-TOF-MS analysis of fraction P2.

Figure 5 Effect of conglycinin peptides on b-galactosidase activities in cecal contents (n = 8). Values are expressed as the mean±SD, ^aP < 0.05 vs control group, ^bP < 0.01 vs conglycinin group.