Screening of hepatocyte proteins binding to F protein of hepatitis C virus by yeast two-hybrid system

Figure 1 Map of “bait” plasmid pGBKT7-F.

Figure 2 Sequences of PCR products A: a 486-bp fragment-F amplified by amplified by RT-PCR; D1: pGBKT7-FBP2; D2: pGADT7-FBP2 cut by EcoRI/RT-PCR; B: pGBKT7-F cut by EcoRI/BamHI; C: a 840-bp fragment-FBP2 BamHI.

Figure 3 Expression of HCV F and HCV FBP2 proteins in yeast. Lane 1: HCV F protein; lane 2: positive control; lane 3: HCV FBP2 protein.

Figure 4 Identification of different colonies by BglII digestion.

Figure 5 Interaction between HCV F protein and FBP2 protein identified by co-immunoprecipitation. Lane 1: HCV F protein; lane 2: interaction between HCV F and FBP₂ proteins.