Effects of Pinus massoniana bark extract on cell proliferation and apoptosis of human hepatoma BEL-7402 cells

Figure 1 Chemical structure of procyanidin B₃.

Figure 2 Dose-dependent effects (A) and time-dependent effects (B) of PMBE on inhibition of BEL-7402 cell growth.

Figure 3 Morphological changes in BEL-7402 cells incubated without PMBE for 24 h (A), with 160 µg/mL PMBE for 24 (B), 48 (C) and 72 h (D). Arrows denote condensed, fragmented nuclei, apoptotic bodies.

Figure 4 Apoptosis induced by PMBE. M: DNA marker DL2000; 1: Treatment with PMBE; 2: Untreated negative control.

Figure 5 Time-course effects and dose–response effects of PMBE on cell cycle distribution (A and B) and apoptosis of BEL-7402 cells (C-E). a: control 24 h; b: 24 h; c: 48 h; d: 72 h.

Figure 6 Expression of Bcl-2 protein in BEL-7402 cells treated with 160 µg/mL PMBE for different time periods. C: control; T: treatment with PMBE; ^aP < 0.05 as significant, ^bP < 0.01 very significant vs the control.