Novel genetic variations of the p53R2 gene in patients with colorectal adenoma and controls

Figure 1 Structural feature and genetic variation analysis of p53R2. A: A representative p53R2 gene structure including a regulatory region with p53 binding sequence within intron 1; B: representative cold SSCP result. p53R2 Regulatory region was amplified, and PCR products were run on a 20% SSCP gel at 15 °C. Lanes 2 and 4 are the heterozygous polymorphism pattern; C: alterations in regulatory region of p53R2 gene: (1) DdeI G/C, and (2) DdeI C/C genotypes; D: Alterations in regulatory region of p53R2 gene: (1) NdeI G/G and (2) A/A genotypes; E: Alterations in regulatory region of p53R2 gene: (1) NdeI G/G and HhaI T/T, and (2) NdeI A/A and HhaI T/C genotypes; F: Alterations in regulatory region of p53R2 gene: 20 bp insertion which replaced an ATTTT between nt 1 831 and nt 1 835.

Figure 2 Representation of p53R2 intron 1. The human p53R2 partial intron 1, as enumerated in the original description of the p53R2 gene (GenBank accession no.: NC000008), is shown. The three variable loci are underlined, boxed 5 bp is replaced by 20 bp nucleotides (5’-GTAAAATACTTCTGTAAATA-3’), the p53 binding sequence is italicized, and forward and reverse primers set in bold.