Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma

Figure 1 Comparison of expression of genes in non-inflamed mucosa of ulcerative colitis (UC) and normal control mucosa. The upper and lower boundaries represent a 2- and 0.5-fold difference in the mean of the expression of each gene between noninflamed UC mucosa and control, respectively. Axis scales are logarithmic.

Figure 2 RT-PCR analysis of HOXa4 gene expression in normal colonic mucosa and UC mucosa (inflamed, non-inflamed). A: Showed RT-PCR was performed on four paired inflamed mucosa (I) and non-inflamed mucosa (NI) samples in UC as well as six normal control mucosa (N1-N6) G3PDH was used as a control for cDNA synthesis; B: Semiquantification of HOXa4 gene expression (HOXa4/G3PDH) was performed in six paired UC mucosa and six normal control mucosa using NIH image software.

Figure 3 RT-PCR analysis of MEF2b gene expression in normal colonic mucosa and UC mucosa (inflamed, non-inflamed). A: Analysis was performed on five normal control mucosa (N1-N5) and three non-inflamed UC mucosa (NI) was performed on four paired inflamed mucosa (I) and non-inflamed mucosa (NI); B: Analysis was performed on four paired inflamed mucosa (I) and non-inflamed mucosa (NI). G3PDH was used as a control for cDNA synthesis; C and D: Semiquantification of MEF2b gene expression (MEF2b/G3PDH) was performed in six paired UC mucosa and six normal control mucosa using NIH image software.

Figure 4 (A and B) RT-PCR analysis of HOXa4 and MEF2b gene expression in eight paired colon cancer (C) and normal colonic mucosa (N). G3PDH was used as a control for cDNA synthesis. (C and D) Semiquantification of HOXa4 and MEF2b gene expression (HOXa4, MEF2b/G3PDH) was performed in eight paired colon cancer and normal colonic mucosa using NIH image software.