Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion

Figure 1 Localization of 8-oxodG and 8-nitroguanine in cancerous and noncancerous liver tissues in an ICC patient with a well-differentiated adenocarcinoma. Formation of 8-oxodG and 8-nitroguanine was assessed by immunohistochemistry using an immunoperoxidase method. Paraffin sections (6 μm thickness) were incubated with a rabbit polyclonal anti-8-nitroguanine antibody and a mouse monoclonal anti-8-oxodG antibody. The original magnification is 200×.

Figure 2 Localization of iNOS and HIF-1α in cancerous liver tissues in an ICC patient with poorly-differentiated adenocarcinoma. The expression of iNOS and HIF-1α was assessed by using double immunofluorescence technique. Paraffin sections were treated with anti-iNOS and anti-HIF-1α antibodies. The original magnification is 400×.

Figure 3 Overall survival curve of ICC patients. Thin line, neural invasion evaluated as -; thick line, neural invasion evaluated as + (Kaplan-Meier method).