Expression and hypermethylation of p27kip1 in hepatocarcinogenesis

Figure 1 Expressions of p27. A: p27 protein was expressed in nuclei of liver cirrhosis (SP ×400); B: p27 protein was expressed in pericancerous cirrhosis, but not in HCC (SP ×200); C: p27mRNA was positive in liver cirrhosis (ISH ×200); D: p27mRNA was positive in pericancerous cirrhosis, but negative in HCC (ISH ×100).

Figure 2 MCP of CpG islands of p27^kip1 promoter. Lane 1: marker; lanes 2, 4, 6, 8 and 10: PCR products of each case to unmethylated specific primers. Lanes 3, 5, 7, 9 and 11: PCR products of each case to methylated specific primers. In HCC19 case, only methylated DNA (M) was present. HCC88 case and L.C21 case (liver cirrhosis case) showed unmethylated statue (U). Positive control (P.C) for methylated DNA was treated with SssI methylase first, normal control (N.C) for unmethylated DNA.