Mutations outside the YMDD motif in the P protein can also cause DHBV resistant to Lamivudine

Figure 1 A: Lamivudine-susceptive DHBV control group; B: LRDHBV control group; C: LRDHBV-infected group administrated by large dose Lamivudine group; D: LRDHBV-infected group administrated by small dose Lamivudine group; E: Lamivudine-susceptive DHBV-infected group administrated by small dose Lamivudine group.

Figure 2 Duck embryo liver cells (5 d after DHBV inoculation, optic microscope × 40). They have no significant effect of Duck embryo liver cells infected with DHBV or LRDHBV.

Figure 3 A: Lamivudine-susceptive DHBV control; B: LRDHBV control; C: LRDHBV-infected cells administrated by large dosage Lamivudine; D: LRDHBV-infected cells administrated by small dosage Lamivudine; E: Lamivudine-susceptive DHBV-infected cells administrated by small dosage Lamivudine.

Figure 4 Electrophoresis graph of the amplification of DHBV complete genome and enzyme cutting of the recombinant. Lanes 1 and 7: λ-EcoT14I digest marker; lane 2: the LAPCR product of DHBV complete genome; lane 3: the cloning product of DHBV complete genome; lane 4: EcoRI enzyme cutting product of DHBV complete genome recombinants; lane 5: loop product of pMD18-T vector; lane 6: EcoRI enzyme cutting product of loop product of pMD18-T vector.

Figure 5 A: KorR86Q mutant point in the P protein of the LRDHBV; B: AorE591T mutant point in the P protein of the LRDHBV.