Screening of hepatocyte proteins binding to complete S protein of hepatitis B virus by yeast-two hybrid system

Figure 1 Structure of “bait” plasmid pGBKT7-complete S.

Figure 2 One thousand three hundred and thirty eight basepair fragment-complete S amplified by RT-PCR (A), pGBKT7-complete S cut by EcoRI/ BamHI (B), a 945 bp fragment-CSBP1 amplified by RT-PCR (C), pGBKT7-CSBP1 and pGADT7-CSBP1 cut by EcoRI/BamHI (D).

Figure 3 Expression of HBV complete S and HBV CSBP1 protein in yeast confirmed by Western blotting. Lane 1: HBV complete S protein; lane 2: positive control; lane 3: HBV CSBP1 protein.

Figure 4 Identification of different colonies with BglII digestion.

Figure 5 Interaction between HBV complete S protein and CSBP1 protein identified by coimmunoprecipitation. Lane 1: HBV complete S protein; lane 2: interaction with two proteins.