Hepatosplenic γδ T-cell lymphoma

Figure 1 Histology changes in case 8. A: medium-sized monomorphic lymphoma cells into the red pulp cords and sinus of the spleen (10×); B: Distinct hepatic sinusoidal infiltrates (20×).

Figure 2 Histological changes and immunophenotypes in case 4. A: Infiltration of lymphoma cells into the red pulp cords and sinus of spleen (40×); B: Distinct hepatic sinusoidal infiltrates (40×); C: Sinusoidal infiltration of lymphoma cells in spleen demonstrated by CD3 (10×); D: TCR γδ immunostaining of Golgi region (40×).

Figure 3 Significant erythrophagocytosis in sinus of the spleen of case 6 (40×).

Figure 4 TCR γ gene PCR products in case 2; Jurkat, lanes 3 and 7: single bands of about 200 bp on 8% PAGE gel; Control, lanes 5 and 8 have no distinctive band.

Figure 5 TCR δ gene PCR products in case 1. Lanes 5-8: single bands of about 120 bp on 10% PAGE gel; control and lanes 3 and 4: no distinctive band (the marker band is 100 bp, and the bands under 100 bp are regarded as unspecific amplification).

Figure 6 Forward and reverse sequencing results of TCR δ gene rearrangements in case 8, verified as products of TCR δ gene rearrangements by searching in BLAST (PubMed).