Kinase domain insert containing receptor promotor controlled suicide gene system kills human umbilical vein endothelial cells

Figure 1 Restriction analysis of pAdtrackKDR-CDglyTK 1. pAdtrackKDR-CDglyTK/(BglII+Xba1); 2. 1kb DNA ladder (products of Dingguo Biotechnology Development Center Co., Ltd.).

Figure 2 PCR amplification of CDglyTK and KDR promotor gene from the recombinant adenovirus DNA. 1. PCR products of the recombinant adenovirus DNA using the upstream and downstream primers of CDglyTK gene; 2. PCR products of the recombinant adenovirus DNA using the upstream and downstream primers of KDR promoter genes; 3. 1 kb DNA ladder (products of Dingguo Biotechnology Development Center Co., Ltd.).

Figure 3 Infection rate of AdKDR-CDglyTK to HUVEC cells.

Figure 4 Recombinant adenovirus infected HUVEC cells GFP expression was visualized by fluorescence microscopy 3 d after HUVEC cells were infected with AdKDR-CdglyTK. A-D: HUVEC cells infected with the recombinant adenoviruses at MOI of 1, 50, 100, and 200, respectively.

Figure 5 Survival rate of transgeneic cells after prodrug administration.

Figure 6 Influence of MOI of the recombinant viruses on the killing effect of prodrugs.