Optimization of competitively differentiated polymerase chain reaction in detection of HBV basal core promoter mutation

Figure 1 Optimization of the concentration of dNTPs. A: optical absorption density, A_m and A_w: A values of wells with horseradish peroxidase-labeled anti-DIG (A_m) and anti-FITC (A_w), respectively, 1: 200 μmol/L of dNTPs, 2: 50 μmol/L of dNTPs, 3: 10 μmol/L of dNTPs, 4: 2 μmol/L of dNTPs.

Figure 2 Detection of BCP mutation in recombinant plasmid DNA using optimized CD-PCR. A: pHB-BCP2 as template, B: pTZ19U-HBV as template, C: A_m/A_w ratio as criteria for the result analysis, D: detection BCP mutation among mixture of pHB-BCP2 and pTZ19U-HBV. A: optical absorption density, A_m and A_w: A values of wells with horseradish peroxidase-labeled anti-DIG (A_m) and anti-FITC (A_w), respectively, 0-100%: copy ratio of pHB-BCP2 with total recombinant plasmid DNA in mixture.