CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R

Figure 1 Morphological change of Bel-7402 during induction of drug-resistance. A: Parent cells; B: morphology change during culture; C: most of the cells died. Some “normal” cell islands appeared.

Figure 2 Survival-ADM concentration curves of three cells. A: Bel-7402/R-2000; B: Bel-7402/R-200; C: Bel-7402/S.

Figure 3 Rhodamine 123 excluding test detected by FCM. A: Control; B: Bel7402/r-2000; C: Bel7402/r-200; D: Bel7402/S.

Figure 4 Indirect immunofluorescence on Pgp detected by FCM. A: Bel7402/s; B: Bel7402/r-200; C: Bel7402/r-2000.

Figure 5 Cytotoxicity of CIK to Bel-7402 at different E:T ratios.

Figure 6 Immunohistochemistry results of the tumor specimen before and after CIK treatment, demonstrated that a large amount of lymphocytes with CD3, CD45, CD45RO, CD68 markers infiltrated in tumor after treatment. A: Immunohistochemistry with anti-CD3 to the specimen before treatment; B: with anti-CD3 to the specimen after treatment; C: immunohistochemistry with anti-CD45 to the specimen before treatment; D: with anti-CD45 to the specimen after treatment; E: immunohistochemistry with anti-CD45RO to the specimen before treatment; F: with anti-CD45RO to the specimen after treatment; G: immunohistochemistry with anti-CD68 to the specimen before treatment; H: with anti-CD68 to the specimen after treatment.