Inhibitory effect of adeno-associated virus-mediated gene transfer of human endostatin on hepatocellular carcinoma

Figure 1 RT-PCR analysis of endostatin in transfected cells. M: DL2000 DNA marker; lane 1: PCR products amplified from rAAV2-hEndo transfected cells; lane 2: PCR products amplified from AAV2 transfected cells.

Figure 2 SDS-PAGE of the supernatant of transfected cells. Lane 1: concentrated supernatant of AAV2 transfected Hep3B cells; lane 2: rAAV2-hEndo transfected Hep3B cells; lane 3: molecular weight standard (Amersham Biosciences), myosin (M_r 220000), phosphorylase (M_r 97000), albumin (M_r 66000), ovalbumin (M_r 45000), carbonic anhydrase (M_r 30000), trypsin inhibitor (M_r 20100), lysozyme (M_r 14300).

Figure 3 Proliferation inhibition of endothelial cells by supernatant from transfected and untransfected cells. ^aP<0.05, ^bP<0.01 and ^dP<0.01 vs AAV2-transfected group.

Figure 4 Immunohistochemical and HE staining of HCC xenograft. A: AAV2-transfected tissue (HE staining, ×200); B: endostatin-transfected tissue (HE staining, ×200); C: MVD of AAV2-transfected tissue (stained with factor VIII-related antigen, ×200); D: MVD of rAAV2-hEndo transfected tissue (stained with factor VIII-related antigen, ×200).