Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase

Figure 1 scFv gene from SOE PCR amplification. Lanes A and F: DNA markers; lanes B-E: SOE PCR products.

Figure 2 plasmid PCR products. A: DNA marker; B and C: PCR products.

Figure 3 SDS-PAGE of ScFv induced products. A: Negative bacteria induced by IPTG; B: low molecular mass protein markers; C: positive bacteria induced by IPTG.

Figure 4 ScFv- pDAP2 plasmid PCR. M: DNA molecular markers; A-G: PCR products.

Figure 5 SDS-PAGE of scFv-AP expressing product. A: Negative bacteria induced by IPTG; B: low molecular mass protein markers; C: positive bacteria induced by IPTG.