Transfection of apoptosis related gene Fas ligand in human hepatocellular carcinoma cells and its significance in apoptosis

Figure 1 Result of PCR and enzyme pCDNA31hisB-hFasL plasmid lines 1, 2: Molecule marker; line 3: EcoRI and BamHI enzyme cut plasmid; lane 4: Uncut plasmid; lane 5: PCR result The sequence of pCDNA31hisB-hFasLwas tested in GenBank Blast.

Figure 2 Expression of FasL in HepG2 cells. A: Transfected FasL cells (×200); B: control cells (×100).

Figure 3 Apoptotic cells at different stages. A: middle and late stage apoptotic cells; B: early, middle and late stage apoptotic cells.